Biotechnology (261)

Paper I Genetic engineering and recombinant DNA technology (36)
S.No. Title Downloads
1 M-01. History of genetic material Click here
2 M-02. DNA modifying enzyme (Nomenclature, Type I-IV) Click here
3 M-03. Restriction endonucleases, Isoschizomers Click here
4 M-04. Classification of Restriction enzymes Click here
5 M-05. DNA Topoisomerase Click here
6 M-06. DNA Methyltransferases Click here
7 M-07. DNA polymerases Click here
8 M-08. DNA replication in Prokrayotes Click here
9 M-09. DNA replication in Eukaryotes Click here
10 M-11. Polymerase chain reaction Click here
11 M-13. PCR based methods for site-directed mutagenesis (Single primer methods viz. Mis-incorporation of mismatched oligos, Over-lap extension) – (Part A) Click here
12 M-14. Evolution in enzymology (Klenow, T7 polymerase, Taq polymerase) Click here
13 M-15. Generalized cloning schemes and strategies Click here
14 M-16. GATEWAY Cloning Click here
15 M-18. Plasmids; Lambda based vectors and derivatives Click here
16 M-19. Insertion vectors, replacement vectors, cosmids, phasmids Click here
17 M-20. Phagemids, in vitro packaging, High-cloning capacity vectors Click here
18 M-21. Single stranded DNA vectors (M13, fd, f1); YACs, BACs, PACs, BIBACs Click here
19 M-22. Central Dogma of life Click here
20 M-23. Understanding the architecture of genes, Gene to genome Click here
21 M-24. Understanding the architecture of Proteins, protein to proteome Click here
22 M-25. Classification of genomics: Functional genomics Click here
23 M-26. Common techniques related to Functional Genomics Click here
24 M-27. Protein Expression Vectors Click here
25 M-29. Protein extraction and purification (protein purification tags, histidine and GST tags, IMAC) Click here
26 M-30. Protein-protein interaction and its requirement, Protein microarray Click here
27 M-31. Techniques involved in protein-protein interaction: immune-precipitation, yeast two-hybrid technique (Y2H), FRET/FRAP, co-localization Click here
28 M-32. Sanger’s Di-deoxy Chain termination method (Use of M13 based ss DNA vectors to cycle sequencing Click here
29 M-33. Plant Transformation vectors Ti-, Ri-plasmids Click here
30 M-34. Binary, Conjugate, selection schemes Click here
31 M-35. Microscopy based Imaging and applications (part-A) Click here
32 M-36. Microscopy based Imaging and applications (part-B) Click here
33 M-37. Microscopy based Imaging and applications (part-C) Click here
34 M-38. Fluorescence In Situ Hybridization (FISH) Click here
35 M-39. Pulse Field Gel Electrophoresis method and application Click here
36 M-40. Gene therapy and its role in disease Click here

Paper II Environmental Biotechnology (38)
S.No. Title Downloads
1 M-01. Basic concepts and environment Click here
2 M-02. Types of environmental pollution Click here
3 M-03. Land pollution arising from high-input agriculture Click here
4 M-04. Air and water pollution and its control Click here
5 M-05. Methodology of environmental management Click here
6 M-06. Waste water treatment Click here
7 M-07. Physical, chemical and biological processes Click here
8 M-08. need for water and natural resource management Click here
9 M-09. bioremediation and biodegradation Click here
10 M-10. Biofuel- gaseous biofuel (Biogas); bio hydrogen and biomethane Click here
11 M-11. bioremediation of soil And water contaminated with pesticides and toxic chemicals Click here
12 M-12. Biodegradation of lignin and cellulose Click here
13 M-13. aerobic processes (activated sludge, oxidation ditches, trickling filter, rotating drums, Click here
14 M-14. anaerobic processes: digestion, filteration, etc Click here
15 M-15. conventional fossil fuels and environmental impact Click here
16 M-16. Renewable and non-Renewable resources of energy Click here
17 M-17. Microbial Biohydrogen production Click here
18 M-18. conversion of sugar to alcohol gasohol Click here
19 M-19. biopesticides; biofertilizers Click here
20 M-20. composting; vermiculture Click here
21 M-21. Treatment schemes of domestic waste and industrial effluents Click here
22 M-22. food, feed and energy from solid waste Click here
23 M-23. biotechnological approaches for management environmental problems. Click here
24 M-24. greenhouse effects, and acid rain;biodiversity and its conservation Click here
25 M-25. General principles for the laboratory and biosafety Click here
26 M-26. National biosafety policies and Regulatory framework Click here
27 M-27. Biotechnological Methods for pollution detection Click here
28 M-28. Biosafety and risk assessment issues and Risk management issues Click here
29 M-29. Biomineralization Click here
30 M-30. Genetically engineered microbes in biotreatment of waste and environmental safety Click here
31 M-31. Health aspects; toxicology, allergenicity Click here
32 M-32. Analysis of bological material and xenobiotics Click here
33 M-33. Role of Probiotics in water quality management Click here
34 M-34. Biotechnological methods in pollution abatement Click here
35 M-35. Convention on biological diversity Click here
36 M-36. Intellectual properties Click here
37 M-37. Implications of IPRS on commercial-lization of biotechnology products. Click here
38 M-38. copyrights, trademarks, trade secrets, patents, geographical indications Click here

Paper III Computational Biology (36)
S.No. Title Downloads
1 M-01. Generating Structural Data And analysis Click here
2 M-02. Sequence Analysis-I : Two sequence alignment Click here
3 M-03. Seuence Analysis-II : Multiple sequence alignment Click here
4 M-04. Database Searching -Text search & BLAST Click here
5 M-05. Pattern Recognition in Sequence Analysis -I Click here
6 M-06. Pattern Recognition in Sequence Analysis -II Click here
7 M-07. Intro to Chemical Databases and Chemoinformatics Click here
8 M-08. Chemical functional descriptors -I Click here
9 M-09. Chemical functional descriptors-II Click here
10 M-10. Relationships between chemical structure and biological activity ( QSAR & QSPR) Click here
11 M-11. ligand based design:pharmacophore generation Click here
12 M-12. ligand based design:pharmacophore based 3DSearching Database Click here
13 M-13. 3D QSAR & Data Driven predictions Click here
14 M-14. Prediction of ADME and Toxicity Click here
15 M-15. Molecular Modelling Click here
16 M-16. Prediction of 2D and 3D structure of Protein -I Click here
17 M-17. Prediction of 2D and 3D structure of Protein -II Click here
18 M-18. Introduction to Force fields Click here
19 M-19. Origin of Force Fields Click here
20 M-20. Introduction to structure based ligand design Click here
21 M-21. SBD and Interaction of Active site Click here
22 M-22. Structure Based design -I Click here
23 M-23. Structure Based design -II Click here
24 M-24. Scoring Click here
25 M-25. Potential Energy Surface and EM Click here
26 M-26. Molecular Simulations and Flexibility in Protein Click here
27 M-27. Solvation model in MD Click here
28 M-28. Application of Solvation model in MD Click here
29 M-29. Methods for calculating free energy and entropy of macromolecular interactions Click here
30 M-30. Introduction to Omics and Genomics,High throughput gene annotation/ Genomics/ GWAS Click here
31 M-31. Proteomics, Transcriptomics and Metabolomics Click here
32 M-32. Metabolic modelling: FBA and MCA approaches Click here
33 M-33. Networks in Biology and Protein-protein interaction networks Click here
34 M-34. Application of tools in target analysis : Infectious disease Click here
35 M-35. Application of Bio &Chemoinformatics tools in designing compounds Click here
36 M-36. Application of Molecular Dynamics in designing compounds Click here

Paper IV Animal Cell Biotechnology (41)
S.No. Title Downloads
1 M-01. History, scope and future perspective of animal cell biotechnology Click here
2 M-02. Laboratory Requirements for Animal Cell Culture Click here
3 M-03. Media and Reagents used in Animal Cell Biotechnology Click here
4 M-04. Instrumentation and analytical techniques Click here
5 M-05. Introduction and history of cell culture, Primary Culture, Secondary Cell Culture and cell lines and applications of animal cell culture Click here
6 M-06. Basic Principal of Animal cell Biotechnology Click here
7 M-07. Organ Culture and application Click here
8 M-08. Cell and tissue engineering Click here
9 M-09. Modifying enzymes and uses in animal cell biotechnology Click here
10 M-10. Transgenic Animal and methods of Creation of Transgenic Animals Click here
11 M-11. Methods for construction of recombinant animal viral vectors for gene transfer into cell lines Click here
12 M-12. Biology of Animal viral vectors Click here
13 M-13. Genetic Engineering and Applications Click here
14 M-14. Manipulation of Gene Expression in Eukaryotes Click here
15 M-15. Collection and Purification Process of Recombinant Proteins Click here
16 M-16. Gene Knockout/targeting Click here
17 M-17. Sperm and Embryo sexing and disease transmission Click here
18 M-18. Pregnancy Diagnosis in Animals Click here
19 M-19. Stem Cell Technology and therapeutics Click here
20 M-20. Assisted Reproductive Technologies Click here
21 M-21. Host Pathogen Interactions Click here
22 M-22. Immunological methods for diagnosis Click here
23 M-23. Nucleic acid based assays Click here
24 M-24. Transcriptomics and proteomics Click here
25 M-25. Metagenomics Click here
26 M-26. DNA sequencing Click here
27 M-27. Animal forensics and applications Click here
28 M-28. Biomarkers for animal disease diagnosis Click here
29 M-29. Production of vaccines and pharmaceutical proteins Click here
30 M-30. Animal cell culture as surrogate for animal usage for drugs and toxic pollutants Click here
31 M-31. Drug Discovery, Development and Risks assessment Click here
32 M-32. Superbugs- problem of modern world Click here
33 M-33. Nanobiotechnology and application Click here
34 M-34. Food safety and dairy biotechnology Click here
35 M-35. Fermentation technology Click here
36 M-36. Cell Cryopreservation and animal Conservation Click here
37 M-37.a. Bioinformatics and Biostatistics P-1 Click here
38 M-37.b. Bioinformatics and Biostatistics P-II Click here
39 M-38. Advances in animal cell biotechnology Click here
40 M-39. Biosafety issues in animal cell biotechnology Click here
41 M-40. Ethical issues for animal cell culture Click here

Paper V Molecular Therapeutics (39)
S.No. Title Downloads
1 M-01. Ethics in biomedical research Click here
2 M-02. Biosafety & biocontainment -I Click here
3 M-03. Biosafety & biocontainment -II Click here
4 M-04. Basics of molecular therapeutics part I Click here
5 M-05. Basics of molecular therapeutics part II Click here
6 M-06. Basics of molecular therapeutics part III Click here
7 M-07. Fundamentals of gene therapy Click here
8 M-08. Gene editing, gene regulation and gene silencing Click here
9 M-09. Cellular and molecular aspects of stem cells Click here
10 M-10. Nuclear reprogramming in molecular theraputics Click here
11 M-11. Identification of drug targets and screening of inhibitors Click here
12 M-12. Molecular mechanism of pathogenesis Click here
13 M-13. Basics of biotherapy Click here
14 M-14. Cellular Therapy Click here
15 M-15. Antibody Therapy Click here
16 M-16. Cytokine therapy Click here
17 M-17. Biology of viral diseases Click here
18 M-18. Biotherapy for viral diseases Click here
19 M-19. Biotherapy of cancer Click here
20 M-21. Biotherapy of bacterial diseases Click here
21 M-22. Biology of fungal infections Click here
22 M-23. Biotherapy of fungal diseases Click here
23 M-24. Biology of protozon dieseases Click here
24 M-25. Biotherapy of protozoan diseases Click here
25 M-26. Research inference and various statistical procedures Click here
26 M-27. Cancer stem cell Click here
27 M-28. Recombinant protein therapeutics Click here
28 M-29. Opioid peptide biotherapeutics Click here
29 M-30. Basics of cancer biology Click here
30 M-31. Basics of mammalian cell culture techniques Click here
31 M-32. frontiers in human genome Click here
32 M-33. Neutrigenomics: The latest trends Click here
33 M-34. Vaccines and vaccinology I Click here
34 M-35. Vaccines and vaccinology II Click here
35 M-36. Animal models in metabolic and infectious diseases Click here
36 M-37. Animal models in molecular therapeutics Click here
37 M-38. Animal Handling and Experimentation Click here
38 M-39. Biostatistics in research Part I Click here
39 M-40. Biostatistics in research Part II Click here

Paper VI Nanobiotechnology (34)
S.No. Title Downloads
1 M-01. Quantification of noncovalent interactions Click here
2 M-02. Noncovalent interactions for self assembly Click here
3 M-03. Hydrogen bonding and hydrophobic interactions Click here
4 M-04. Cooperativity- synchronizing noncovalent interactions Click here
5 M-05. Self assembly in nature: micelles, liposomes and capsules Click here
6 M-06. Hierarchical structures peptides and proteins Click here
7 M-07. Nanostructures of peptides and proteins Click here
8 M-08. Understanding structures and interactions in nucleic acids Click here
9 M-09. Design of nanostructures from nucleic acids Click here
10 M-10. Nanostructures from hybrid systems Click here
11 M-11. Nanotechnology, nanomedicine and nanomaterials: applications in biotechnology Click here
12 M-12. Concept of targeting: role of Nanotechnology Click here
13 M-13. Nanotechnology based delivery systems for biotechnological applications Click here
14 M-14. Biotechnological applications of hyperbranched polymers (including dendrimers) based nanomaterials Click here
15 M-15. Quantum dots: role in diagnosis and implication in drug delivery Click here
16 M-16. Carbon based nanomaterials Click here
17 M-17. Theranostic applications: recent trends in biotechnological applications of nanomaterials Click here
18 M-24. Environmental remediation related nanoparticles, their synthesis, design, reactivity and applications in removal of various pollutants Click here
19 M-25. Use of nanoparticles in water purification Click here
20 M-26. Design of nanoparticles as a new approach to solve the issue of biofouling Click here
21 M-27. Air pollution control using nanoparticles Click here
22 M-28. Possible risks posed by nanotechnology Click here
23 M-29. Challenges assosiated with the development of cost effective and environmentally acceptable functional nanomaterials for control of pollution) Click here
24 M-30. Future trends for pollutant removal: nanocleansing and nanobioremediation Click here
25 M-31. Medical Nanobiotechnology: Nanodiagnostic tools Click here
26 M-32. Medical Nanobiotechnology: Management of cancer Click here
27 M-33. Medical Nanobiotechnology: Nanoscience based sample extraction techniques Click here
28 M-34. Medical Nanobiotechnology: Analysis of nanoscience based materials Click here
29 M-35. Food Nanotechnology: an introduction Click here
30 M-36. Nanobiosensors for food safety I: electrochemical Click here
31 M-37. Nanobiosensors for food safety II: optical Click here
32 M-38. Functional foods and nanotechnological interventions I: Nutraceuticals and their limitations Click here
33 M-39. Functional foods and nanotechnological interventions II: Nanoformulations for value addition Click here
34 M-40. Nanotechnology and food packages Click here

Paper VII Plant biotechnology and crop improvement (37)
S.No. Title Downloads
1 M-01. Introduction to cropping systems in India Click here
2 M-02. Set up Tissue Culture Laboratory Click here
3 M-03. History/background of plant tissue culture Click here
4 M-04. Medium constituents (macro-and microelements, hormones, other growth regulators) and preparation Click here
5 M-05. Cell & Tissue culture Click here
6 M-06. Micropropagation-I Click here
7 M-07. Micropropagation Shoot multiplication Click here
8 M-09. Common problems associated with acclimatization / Commercial Applications Click here
9 M-10. Laboratory for PTC Click here
10 M-11. Somatic Embryogenesis Click here
11 M-12. Organogenesis Click here
12 M-13. In Vitro Production of Haploids-Part 1 (Anther Culture) Click here
13 M-14. In Vitro Production of Haploids-Part 2 (Gynogenesis Culture) Click here
14 M-15. In Vitro Production of Triploids Click here
15 M-16. Zygotic embryo culture Click here
16 M-17. Somatic Hybridization, culture and fusion: selection of hybrid cells and regeneration of hybrid plants Click here
17 M-18. Production of secondary metabolites Click here
18 M-19. In situ conservation and cryopreservation Click here
19 M-20. Production of virus-free plants Click here
20 M-21. Conventional breeding versus Transgenesis Click here
21 M-22. Introduction to Agrobacterium tumefaciens and A. rhizogene Click here
22 M-23. Features of Ti and Ri Plasmids and their use as vectors, Binary and co-integrate vectors Click here
23 M-24. Cloning strategies Click here
24 M-25. Methods used in DNA delivery Click here
25 M-26. Agrobacterium mediated transformation Click here
26 M-27. Direct DNA transfer to plants (particle gun, electroporation, Silicon carbide fibres, PEG mediated, in-planta) Click here
27 M-28. Chloroplast Transformation 1 Click here
28 M-29. Chloroplast Transformation II Click here
29 M-30. DNA viruses as Expression vectors (Cauliflower mosaic virus, Gemini virus, Tobacco mosaic virus, Potato X virus) Click here
30 M-31. Detection, characterization and expression of transformants (Genetic markers, reporter genes, transgene stability and gene silencing) Click here
31 M-32. GM technology for :Conferring resistance to biotic stresses (pests, viruses, fungi) Click here
32 M-33. GM technology for conferring resistance to Abiotic stresses (tolerance to salt, cold, drought) Click here
33 M-34. GM technology for Herbicide Click here
34 M-35. Increasing shelf life of fruits and flowers Click here
35 M-36. Enhancing the nutritional quality Click here
36 M-37. Metabolic engineering and industrial products (plant secondary metabolities, alkaloids, industrial enzymes, therapeutic proteins, antibodies, edible vaccines) Click here
37 M-38. Aspects related to commercial release of transgenic crops Environmental and Biosafety Issues and Regulatory considerations for Modern Biotechnology Click here